|Become fully competent in processing lymphoid and hematopoietic tissues.
- Proper triaging of the tissue for routine histopathology, immunophenotyping, cytogenetics, and molecular genetic analysis.
- Understanding the importance of tissue also as a key resource for investigational studies and for evaluating the efficacy of future treatment modalities.
|To perform bone marrow biopsies and bone marrow aspirations.
- The actual procedure.
- Ordering/performing the appropriate stains on smear slide preparations (Wright–Giemsa staining or other Romanowsky–type of staining, and special stains, such as Iron stain, MPO, etc.)
- Accurately evaluating and interpreting them.
|Perform, evaluate and correctly interpret a variety of laboratory results.
- Flow cytometry immunophenotypic data.
- Automated CBC results, such as RBC, WBC and platelet counts, as well as hemoglobin concentrations.
- Automated and manual differential white blood cell counts.
- Routine coagulation tests (PT, PTT, fibrinogen, thrombin time).
- Tests for hemoglobinopathies.
- Work–ups of patients with platelet dysfunctions.
|Develop expertise in the correct morphologic identification of hematopoietic and lymphoid proliferations.
- Distinction of hemato/lymphoid cells from non–hematopoietic cell types.
- Distinction of benign from malignant hemato/lymphoid cells.
- Recognition of the influences of processing (different fixatives, freezing artifacts, etc.) on the morphologic appearances of different cell types.
|Evaluate bone marrow aspirate and peripheral blood smears and recognize the variety of cell types and stages of maturation.
- Bone marrow differential counts.
- Evaluation of red cell and white cell morphology.
- Identification of malignant and hematopoietic cell populations.
|Be familiar with the concepts and principles of gene product expression and detection, and to understand the current applications of molecular biology in diagnostic pathology.
|Be able to coordinate all the pathological information obtained in order to reach a final diagnostic conclusion.
- Developing skills in correlating gross, microscopic, immunophenotypic and molecular genetic findings.
- Evaluating all of these pathologic parameters in the context of the clinical, radiographic and other laboratory findings.
- Understanding the role and limitations of routine morphology, immunophenotyping and molecular/genetic techniques and therefore recognizing the appropriate order of these studies in a diagnostic laboratory.